Investigation of parasite genetic variation and systemic immune responses in patients presenting with different clinical presentations of cutaneous leishmaniasis caused by Leishmania aethiopica

作者：Endalew Yizengaw, Yegnasew Takele, Susanne U. Franssen, Bizuayehu Gashaw, Mulat Yimer, Emebet Adem, Endalkachew Nibret, Gizachew Yismaw, Edward Cruz Cervera, Kefale Ejigu, Dessalegn Tamiru, Abaineh Munshea, Ingrid Müller, Richard Weller, James A. Cotton, Pascale Kropf · 发表于：Infectious Diseases of Poverty · 年份：2024 · DOI：10.1186/s40249-024-01244-x · 被引用次数：12 · 研究领域：Research on Leishmaniasis Studies、Trypanosoma species research and implications、Parasites and Host Interactions

BACKGROUND: Cutaneous leishmaniasis (CL) is a neglected tropical skin disease, caused by the protozoan parasite Leishmania. In Ethiopia, CL is mainly caused by Leishmania aethiopica and can present in different clinical forms. The aim of this study was to assess whether these different forms are associated with differences in parasite genetic and host systemic immune signatures. METHODS: Here we analysed the whole genome sequence data for 48 clinical parasite isolates and the systemic immune signature from a cohort of CL patients, who were recruited in Nefas Mewcha, Northern Ethiopia, from January 2019 to January 2022. RESULTS: Our results show that parasites from CL cases with different presentations in a single Ethiopian setting are from the same genetic population based on a permutation test of genome-wide similarity. Furthermore, a logistic regression test for genome wide association did not identify any individual genetic variants significantly associated with disease presentation. We also measured plasma chemokine and cytokine levels of 129 CL patients presenting with different forms of CL. None of the chemokine [eotaxin, eotaxin-3, interleukin (IL)-8, interferon (IFN)-γ-induced protein-10 (IP-10), monocyte chemoattractant protein (MCP)-1, MCP-4, macrophage-derived chemokines (MDC), macrophage inflammatory protein (MIP)-1α, MIP-1β and thymus- and activation-regulated chemokine (TARC)] or cytokine (IFN-γ, IL-1β, interleukin-2, IL-4, IL-6, IL-10, IL-12p70, IL-13, tumor ne...