Characterization of the emerging recombinant infectious bronchitis virus in China

作者：Suchun Wang, Junhui Pan, Kaiyutai Zhou, Dianfeng Chu, Jinji Li, Yiping Chen, Qian Qi, Shimeng Wei, Chao Li, Jinyu Sui, Faxing Wu, Jinping Li, Guangyu Hou, Hualei Liu, Kaicheng Wang · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1456415 · 被引用次数：8 · 研究领域：Animal Virus Infections Studies、Viral gastroenteritis research and epidemiology、Plant Virus Research Studies

Infectious bronchitis virus (IBV) can cause serious harm to poultry industry. It is belong to Coronaviridae which is highly variable. A kind of emerging recombinant IBV (ahysx-1) has been detected in chicken from China in 2016. To understand the epidemiology and characterization of the emerging recombinant IBV, 35,455 samples of chickens from the 15 provinces in China were collected and detected. One hundred and ninety-six out of the 537 flocks (positive rate, 36.49%), and 908 out of 35,455 samples (positive rate, 2.56%) were positive in the detection. The results showed that the emerging recombinant IBV was pandemic in China. Thirteen emerging recombinant IBV isolates were selected and continuous subcultured to the fourth generation and analyzed by Next-generation sequencing. Compared with the reported sequence of ahysx-1, the genomic analysis showed that multiple position insertions and deletions were in 1a gene, 3b gene, M gene and N gene. The identity of the S gene nucleotide sequence between all the 13 emerging recombinant IBV isolates and reference stain ahysx-1 were 98.1–99.1%, while the identity of amino acid sequence were 98.0–99.8%. To better understand the recombination mechanism of the emerging recombinant IBV, the genomic sequence of the 13 isolates were compared with turkey coronavirus or guinea fowl coronavirus. The results suggest that all the 13 emerging recombinant IBV isolates were likely to be the recombination of turkey coronavirus or guinea fowl coronavi...