Single-cell transcriptomic profiling uncovers cellular complexity and microenvironment in gastric tumorigenesis associated with Helicobacter pylori

作者：Nianshuang Li, Sihai Chen, Xinbo Xu, Huan Wang, Pan Zheng, Fei Xiao, Huajing Ke, Yuting Lei, Yanan Zhou, Xiaoyu Yang, Yaobin Ouyang, Chuan Xie, Cong He, Yi Hu, Yi Cao, Zhengrong Li, Yong Xie, Zhongming Ge, Xu Shu, Nonghua Lü, Jianping Liu, Yin Zhu · 发表于：Journal of Advanced Research · 年份：2024 · DOI：10.1016/j.jare.2024.10.012 · 被引用次数：37 · 研究领域：Single-cell and spatial transcriptomics、Immune cells in cancer、Helicobacter pylori-related gastroenterology studies

Graphical summary of cellular heterogeneity and microenvironment along the consecutive gastric carcinogenesis associated with H. pylori. (A) Cellular heterogeneity along the histological cascade of gastric carcinogenesis with or without H. pylori infection. (B) The DEGs between H. pylori −positive and −negative cell types. GS, gastritis; IM, intestinal metaplasia; GC, gastric cancer. • ScRNA-seq data revealed cellular heterogeneity in gastric tumorigenesis. • Enterocytes, was overwhelmingly abundant in gastric intestinal metaplasia lesion. HNF4G was predicted as the specific transcription factor. • ScRNA-seq unveiled the transcriptional features of distinct cell subtypes in GC. • The differentia expressed genes (DEGs) in epithelial, fibroblasts and myeolid cells associated with H. pylori infection have been identified. • H. pylori -positive specimens exhibited enriched cell-cell communication, with significant active TNF signaling network. Helicobacter pylori ( H. pylori ) infection is the main risk for gastric cancer (GC). However, the cellular heterogeneity and underlying molecular mechanisms in H. pylori -driven gastric tumorigenesis are poorly understood. Here, we generated a single-cell atlas of gastric tumorigenesis comprising 18 specimens of gastritis, gastric intestinal metaplasia (IM) and GC with or without H. pylori infection. Single-cell RNA sequencing (scRNA-seq) was performed. Immunofluorescence, immunohistochemistry and qRT-PCR analysis were applied in a second ...