The association between non-alcoholic liver fatty disease and coronary artery calcification: a two-sample Mendelian randomization study

作者：Liaoming He, Xieraili Tiemuerniyazi, Ziang Yang, Shengkang Huang, Lianxin Chen, Yifeng Nan, Yangwu Song, Wei Feng · 发表于：European Journal of Preventive Cardiology · 年份：2024 · DOI：10.1093/eurjpc/zwae336 · 被引用次数：7 · 研究领域：Liver Disease Diagnosis and Treatment、Genetic Associations and Epidemiology、Cardiovascular Disease and Adiposity

BACKGROUND: Although prior observational studies have suggested that patients with non-alcohol fatty liver disease (NAFLD) may have a higher risk of coronary artery calcification (CAC), these findings remain controversial. This study aimed to explore the causal association between NAFLD and CAC at genetic level by two-sample Mendelian randomization (MR) analysis. METHOD: Utilizing summary-level data from multiple large-scale genome-wide association studies (GWAS) in European populations, a two-sample MR analysis was initially conducted to explore the potential causal association between NAFLD and CAC. The results of the MR analysis were pooled through random-effect meta-analysis. The inverse variance weighting (IVW) method served as the primary approach for MR analysis. Additionally, the weighted median, MR-Egger and MR-pleiotropy residual sum and outlier (MR-PRESSO) methods was applied for sensitivity analysis. Summary-level data on liver fatty content was utilized for validation analysis, while summary-level data on cirrhosis served as positive control, further ensuring the validity and robustness of our findings. Reverse MR analysis was performed to assess the association between CAC and NAFLD, employing instrument variables derived from CAC. RESULTS: The MR analysis indicated that genetically predicted NAFLD had no effects on the risk of CAC (Beta: 0.01, 95% CI: -0.02 to 0.03, P = 0.74). Likewise, the reverse MR analysis found no significant genetic association between CA...