Mtb/HIV co-infection immune microenvironment subpopulations heterogeneity

作者：Jiamin Gao, Xianzhen Huang, Qingdong Zhu, Huawei He, Jie Zhang, Jieling Chen, Cailing Wei, Shunda Luo, Shixiong Yang, Zhouhua Xie · 发表于：International Immunopharmacology · 年份：2024 · DOI：10.1016/j.intimp.2024.113341 · 被引用次数：4 · 研究领域：Single-cell and spatial transcriptomics、Tuberculosis Research and Epidemiology、HIV Research and Treatment

• Single-cell RNA analysis reveals insights into HIV and Mtb/HIV co-infection dynamics. • CD4 + T_RACK1_STAT1 subpopulation significantly enriched in Jak-STAT signaling pathway • HIV and Mtb/HIV -specific CTL_GNLY and CD8 + T_RACK1_TIGIT subpopulations identified. • Unique monocyte subpopulations suggest differing infection states and functions. • NK_HSPA1A and NK_NEAT1 subpopulations play a crucial role in immune response. The co-infection of human immunodeficiency virus type 1 (HIV-1) and tuberculosis poses a lethal threat. Currently, our understanding of the altered immune responses and diverse immune cell subpopulations triggered by dual pathogen infections remains inadequate. We utilized single-cell RNA sequencing data from the Gene Expression Omnibus database and the China National GeneBank Nucleotide Sequence Archive to study peripheral blood mononuclear cells from individuals infected with HIV-1 and those co-infected with Mycobacterium tuberculosis (Mtb)/HIV. We investigated cellular components, signaling pathways, biological functions, developmental trajectories, and gene regulatory networks among different cells to determine cellular heterogeneity in the progression of Mtb/HIV co-infection. We constructed a single-cell global transcriptional landscape of Mtb/HIV co-infection, revealing heterogeneity among various cell subpopulations. CD4 + T_RACK1_STAT1 subpopulation may participate in the JAK-STAT signaling pathway through RACK1-mediated transcriptional regulation ...