Plasmodesmata act as unconventional membrane contact sites regulating inter-cellular molecular exchange in plants

作者：Jessica Pérez‐Sancho, Marija Smokvarska, Gwennogan Dubois, Marie Glavier, Sujith Sritharan, Tatiana Sousa Moraes, Hortense Moreau, V. Dietrich, Matthieu Pierre Platre, Andrea Paterlini, Ziqiang Patrick Li, Laëtitia Fouillen, Magali Grison, Pepe Cana‐Quijada, Françoise Immel, Valérie Wattelet, Mathieu Ducros, Lysiane Brocard, Clément Chambaud, Yongming Luo, Priya Ramakrishna, Vincent Bayle, Linnka Legendre-Lefebvre, Stéphane Claverol, Matej Zábrady, Wolfgang Busch, Marie Barberon, Jens Tilsner, Ykä Helariutta, Eugenia Russinova, Antoine Taly, Yvon Jaillais, Emmanuelle Bayer · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2023 · DOI：10.1101/2023.12.18.572149 · 被引用次数：9 · 研究领域：Plant nutrient uptake and metabolism、Legume Nitrogen Fixing Symbiosis、Phytase and its Applications

SUMMARY Membrane contact sites (MCS) are fundamental for intracellular communication, but their role in intercellular communication remains unexplored. We show that in plants, plasmodesmata communication bridges function as atypical endoplasmic reticulum (ER)-plasma membrane (PM) tubular MCS, operating at cell-cell interfaces. Similar to other MCS, ER-PM apposition is controlled by a protein-lipid tethering complex, but uniquely, this serves intercellular communication. Combining high-resolution microscopy, molecular dynamics, pharmacological and genetic approaches, we show that cell-cell trafficking is modulated through the combined action of Multiple C2 domains and transmembrane domain proteins (MCTP) 3, 4, and 6 ER-PM tethers, and phosphatidylinositol-4-phosphate (PI4P) lipid. Graded PI4P amounts regulate MCTP docking to the PM, their plasmodesmata localization and cell-cell permeability. SAC7, an ER-localized PI4P-phosphatase, regulates MCTP4 accumulation at plasmodesmata and modulates cell-cell trafficking capacity in a cell-type specific manner. Our findings expand MCS’s functions in information transmission, from intracellular to intercellular cellular activities. In brief Plant intercellular communication is regulated via tubular membrane contact through PI4P binding-ER-PM tether MCTP proteins Highlights Plasmodesmata are unconventional ER/PM tubular contact sites located at cell-cell interface Plasmodesmata operate as control valves, modulating ER-PM contacts to regu...