Phytohormonal dynamics in the abscission zone of Korla fragrant pear during calyx abscission: a visual study

作者：Lingling Zheng, Yue Wen, Lin Yan, Jia Tian, Junjie Shaobai, Zhichao Hao, Chunfeng Wang, Tianyu Sun, Lei Wang, Chen Chen · 发表于：Frontiers in Plant Science · 年份：2024 · DOI：10.3389/fpls.2024.1452072 · 被引用次数：9 · 研究领域：Plant Physiology and Cultivation Studies、Plant and animal studies、Plant Reproductive Biology

Introduction Phytohormones play a crucial role in regulating the abscission of plant organs and tissues. Methods In this study, the ultrastructure of the sepals of Korla fragrant pears was observed using a transmission electron microscope, and high-performance liquid and gas chromatography were used to analyze the dynamic changes of phytohormones in the abscission zone during the calyx abscission process of Korla fragrant pears, and mass spectrometry imaging was applied to ascertain the spatial distribution of phytohormones. Results The results revealed that the mitochondria in the abscission zone of the decalyx fruits were regularly distributed around the cell wall, and the chloroplasts were moderately present. In contrast, in the persistent calyx fruit, the corresponding parts of the abscission zone showed a scattered distribution of mitochondria within the cells, and there was a higher number of chloroplasts, which also contained starch granules inside. Mass spectrometry imaging revealed that ABA was enriched in the abscission zone of the decalyx fruit, and their ionic signal intensities were significantly stronger than those of the persistent calyx fruit. However, the ionic signal intensities of Indole-3-acetic acid (IAA) and Gibberellin A3 (GA 3 ) of the persistent calyx fruit were significantly stronger than those in the abscission zone of the decalyx fruit and were concentrated in the persistent calyx fruit. 1-Aminocyclopropanecarboxylic Acid (ACC) did not show distinc...