Shift in potential pathogenic bacteria during permafrost degradation on the Qinghai-Tibet Plateau

作者：Binglin Zhang, Baogui Zhang, Yeteng Xu, Xiao Yan, Shijin Wang, Xing‐Lou Yang, Hang Yang, Gaosen Zhang, Wei Zhang, Tuo Chen, Guangxiu Liu · 发表于：The Science of The Total Environment · 年份：2024 · DOI：10.1016/j.scitotenv.2024.176778 · 被引用次数：7 · 研究领域：Climate change and permafrost、Polar Research and Ecology、Indigenous Studies and Ecology

Permafrost acts as a potential pathogen reservoir. With accelerating climate change and intensifying permafrost degradation, the release of these pathogens poses significant threats to ecosystems and public health. However, the changes in pathogenic communities during permafrost degradation remain unclear. This study utilized quantitative PCR and Illumina high-throughput sequencing to analyze the composition and quantities of potential pathogenic bacteria in four types of permafrost soil on the northeast edge of the Qinghai-Tibet Plateau (QTP): sub-stable permafrost (SSP), transition permafrost (TP), unstable permafrost (UP), and extremely unstable permafrost (EUP). The results showed that during permafrost degradation, the quantity of potential pathogenic bacteria decreased from 7.8 × 10 6 to 3.1 × 10 6 copies/g. Both the Richness and Shannon indices initially declined from SSP, to TP, UP, and then began to rise when permafrost degraded to EUP. A total of 216 potential pathogenic bacterial species were identified, including 166 animal pathogens , 28 zoonotic pathogens, and 22 plant pathogens . The pathogenic community intergroup differences (ANOSIM), unique taxa, and dominant pathogen analysis indicated the significant changes in pathogenic communities during permafrost degradation. The potential pathogenic community was significantly influenced by non-pathogenic bacterial communities (Procrustes analysis), with soil moisture being the primary environmental factor, followed ...