Importance of Ultraviolet-C (UV-C) Emitter Configuration for Clostridioides difficile Attenuation

作者：Carmen T Brindeiro, Franklin Dexter, Michelle C. Parra, Kaitlin M. Walker, Soyun M Hwang, Brendan T. Wanta, Debra J. Szeluga, Brent Hadder, Melinda Seering, Jonathan E. Charnin, Randy W. Loftus · 发表于：Cureus · 年份：2024 · DOI：10.7759/cureus.71096 · 被引用次数：2 · 研究领域：Clostridium difficile and Clostridium perfringens research、Infection Control in Healthcare、Nosocomial Infections in ICU

Background: The impact of ultraviolet-C (UV-C) emitter configuration on pathogen attenuation has not been assessed. We hypothesized that emitter configuration would impact UV-C efficacy for Clostridioides difficile (C. difficile) attenuation. Methods: C. difficile carriers (ReadyNowTM Test Carriers, Stratix Labs Corporation, Saint Paul, MN) inoculated with > 108 C. difficile American Type Culture Collection (ATCC) 43593 (according to American Society for Testing and Materials (ASTM) 3135 standards) were obtained, and the following experiments were conducted from April to August of 2023. Each of the three carriers along with three calibrated radiometers (ILT1270, International Light Technologies, Peabody, MA) were mounted on an aluminum stand at positions A (left of center of stand), B (center of stand), and C (right of center of stand). The stand was positioned at 9 feet (2.74 m) from and directly ahead of UV-C emitters utilizing low-pressure mercury gas UV-C lamps (Surfacide, Waukesha, WI). Five UV-C emitter configurations were assessed; (1) three emitters with a triangular configuration about the stand and each rotating 360 degrees, (2) one emitter facing the stand and rotating 360 degrees, (3) three emitters facing the stand in a linear configuration and each rotating 5 degrees, (4) one emitter facing the stand and rotating 5 degrees, and (5) one emitter facing the stand and rotating 90 degrees. Three serial experiments were conducted. The first experiment used a dose t...