Magnetic resonance imaging classification in a percutaneous needle injury rat model of intervertebral disc degeneration

作者：Songlin Liang, Nianhu Li, Jiawen Zhan, Zhichao Li, Changjun Tie, Yanjie Zhu, Hongyan Guo, Liqing Ke, Jian Li, Zhanwang Xu, Peng Zhang, Wenxiang Cheng · 发表于：Journal of Orthopaedic Surgery and Research · 年份：2024 · DOI：10.1186/s13018-024-05110-2 · 被引用次数：8 · 研究领域：Spine and Intervertebral Disc Pathology、Cervical and Thoracic Myelopathy、Pain Mechanisms and Treatments

BACKGROUND: During the development of disease-modifying intervertebral disc degeneration (IDD) drugs, the rat model of IDD is frequently used for disease progression assessment. The aim of this study was to describe a magnetic resonance (MRI) scoring system for the assessment of different disc conditions in puncture-induced IDD, allowing standardization and comparison of results obtained by different investigators. METHODS: A total of 36 Sprague-Dawley rats were utilized in the present study. The animals were divided into two groups: a sham group and an IDD group caused by puncture. The rats in the IDD group were subsequently divided into six categories based on time frames, with five rats in each category. The sham group was divided into two sub-groups (n = 3) for 28 and 56 days, respectively. T2-weighted images of rats consecutively studied with MRI of the coccygeal discs were classified according to the time course using the corresponding histological data. Additional scoring of the micro-CT was employed to identify the progression of bone destruction of the rat model of IDD. RESULTS: A comparison of the MRI results between the sham group and the IDD group revealed a significant reduction in NP height, area, T2WI value, and DHI in the latter group (P < 0.05). The micro-CT results demonstrated that following acupuncture, there was a notable decline in the BV, Tb.N, and height of the coccygeal vertebra, while the BS/BV and Tb.Sp exhibited a significant increase (P < 0.05). T...