Piezo1 Promotes Osteogenesis Through C a MKII Signalling in a Rat Maxillary Expansion Model

作者：Zhengquan He, Yugui Du, Chuhan Peng, Bowen Xu, Jiayue Tang, R M Liu, Kai Yang · 发表于：Orthodontics and Craniofacial Research · 年份：2024 · DOI：10.1111/ocr.12862 · 被引用次数：9 · 研究领域：Erythrocyte Function and Pathophysiology、Planarian Biology and Electrostimulation、Hydrogen's biological and therapeutic effects

OBJECTIVES: Rapid maxillary expansion (RME) is a widely used technique to treat maxillary transverse deficiency. Piezo1 is a cation channel that is activated by mechanical force and regulates bone formation. This study aims to elucidate the role of Piezo1 in bone remodelling during the RME process. MATERIALS AND METHODS: In this study, the periosteal-derived stem cells (PDSCs) were cultured and stretched by the Flexcell system. The effects of Piezo1 on osteogenesis were assessed via RNA sequencing, real-time quantitative PCR, and western blot analyses. Moreover, for in vivo analyses, the rat RME model was established. The function of Piezo1 in mid-palatal suture bone remodelling was evaluated using micro-CT, haematoxylin-eosin (HE) staining, and immunohistochemistry analyses. RESULTS: /calmodulin -dependent protein kinase (CaMKII) were significantly enhanced in PDSCs over time. Furthermore, these were also upregulated in the RME model with the expansion of the mid-palatal suture. However, Piezo1 inhibition by Grammostola spatulata mechanotoxin 4 downregulated the levels of these factors in the RME model. CONCLUSIONS: This study indicated that Piezo1 is associated with the osteogenesis of PDSCs and bone remodelling in the RME process. CaMKII might also participate in this process.