Genome‐Based Molecular Diversity of Extended‐Spectrum β ‐Lactamase‐Producing Escherichia coli From Pigeons in China

作者：Shuangyu Li, Xinshuai Liu, Haoyu Zhao, Yuhua Zhang, Lu Zheng, Juan Wang, Ruichao Li, Peng Xie, Yibin Hu, Caiyuan Zhou, Qian Mao, Leilei Sun, Shanshan Li, Wenhui Wang, Fang Wang, Xinyu Liu, Tiantian Liu, Wei Pan, Chengbao Wang · 发表于：Transboundary and Emerging Diseases · 年份：2024 · DOI：10.1155/2024/1828830 · 被引用次数：2 · 研究领域：Antibiotic Resistance in Bacteria、Escherichia coli research studies、Bacterial Identification and Susceptibility Testing

Extended‐spectrum β ‐lactamase‐producing Escherichia coli (ESBL‐EC) strains present a significant menace to the well‐being of both animals and humans. However, limited information is available regarding their profiles in pigeons. Using a combination of whole genome sequencing, drug susceptibility testing, and bioinformatics analysis, we examined the genomic features and epidemiology of 95 ESBL‐EC strains (41 racing and 54 meat pigeons) that were isolated from 11 Chinese cities. These strains belonged to seven phylogenetic groups (A, B1, B2, C, D, E, and F). Moreover, these isolates have 51 serotypes, including several pathogenic ones (e.g., O51, O8, O4, O25, and O6). Notably, two high‐risk clones, ST131 O25:H4, were found in racing pigeons and were responsible for the worldwide outbreaks of highly pathogenic and multidrug‐resistant (MDR) E. coli infections. In addition, we found 41 multilocus sequence typing types, of which the dominant types were ST155, ST20, ST1011, and ST1196. In total, 91 isolates (95.79%) showed MDR, while eight isolates (8.42%) showed resistance to up to eight classes of antibiotics. Furthermore, we identified a series of ESBL genes in these isolates, including bla CTX-M , bla TEM , bla OXA , bla LAP , and bla CMY . Also, 50 other antibiotic resistance genes (ARGs) were accompanied by the carriage of 33 plasmid replicon types, facilitating the horizontal spread of ARGs. Interestingly, three mcr-1 , four mcr-1.1 , and one tet (X4) were found in isolates ...