Enhanced carbon use efficiency and warming resistance of soil microorganisms under organic amendment

作者：Ling Li, Chenhua Li, Hanyue Guo, Yunhua Liu, Jiandong Sheng, Shiwei Guo, Qirong Shen, Ning Ling, Junjie Guo · 发表于：Environment International · 年份：2024 · DOI：10.1016/j.envint.2024.109043 · 被引用次数：13 · 研究领域：Soil Carbon and Nitrogen Dynamics、Legume Nitrogen Fixing Symbiosis、Plant Parasitism and Resistance

• Microbial CUE and growing taxa response to temperature changes were studied. • Organic amendments increased microbial CUE and resistance to high temperatures. • Bacteria rather than fungi drive microbial CUE responses to temperature. The frequency and intensity of extreme weather events, including rapid temperature fluctuations, are increasing because of climate change. Long-term fertilization practices have been observed to alter microbial physiology and community structure, thereby affecting soil carbon sequestration. However, the effects of warming on the carbon sequestration potential of soil microbes adapted to long-term fertilization remain poorly understood. In this study, we utilized 18 O isotope labeling to assess microbial carbon use efficiency (CUE) and employed stable isotope probing (SIP) with 18 O-H 2 O to identify growing taxa in response to temperature changes (5–35 °C). Organic amendment with manure or straw residue significantly increased microbial CUE by 86–181 % compared to unfertilized soils. The microorganisms inhabiting organic amended soils displayed greater resistance of microbial CUE to high temperatures (25–35 °C) compared to those inhabiting soils fertilized only with minerals. Microbial growth patterns determined by the classification of taxa into incorporators or non-incorporators based on 18 O incorporation into DNA exhibited limited phylogenetic conservation in response to temperature changes. Microbial clusters were identified by grouping ta...