Transcriptome analysis of the coexpression network of genes related to antioxidant characteristics after grain filling in purple rice

作者：Changhui Sun, Junkai Zhu, Ying Zhu, Jinxia Cao, Jiao Zhang, Yanqiong Zhang, Huijuan Zhou, Yangang Zhu, Yiming Ji, Rui Ding, Qiangqiang Xiong, Xiaobin Liu · 发表于：Scientific Reports · 年份：2024 · DOI：10.1038/s41598-024-73698-w · 被引用次数：4 · 研究领域：GABA and Rice Research、Microbial Metabolism and Applications、Food composition and properties

Antioxidant capacity is an important indicator for evaluating the growth and developmental quality of rice. This study has guiding significance for the cultivation of high-nutrient-value varieties. To investigate the molecular mechanisms underlying the antioxidant characteristics of rice grains after the filling stage, Yangzinuo 1 (YZN1) was used as the experimental material, and grains collected at five different time points (7 days apart) after the filling stage were used for transcriptome sequencing. Through weighted gene coexpression network analysis (WGCNA), a coexpression network of gene weights related to antioxidant characteristics was constructed. LOC_Os10g39140, LOC_Os10g38276, and LOC_Os05g45740 were identified from the 2 modules showing the highest correlations with the target traits. GO functional annotation showed that target modules were enriched in pathways related to phenylalanine, flavonoids, and other related pathways, such as GO:0006558, GO:0006559, GO:0009812, and GO:0009813. Correlation analysis with metabolites revealed that differentially expressed genes were significantly enriched in pathways related to antioxidant characteristics and energy metabolism processes, such as glycolysis/gluconeogenesis and flavonoid biosynthesis. The core genes identified in this study were found to be highly correlated with antioxidant characteristics and enriched in pathways related to metabolic and energy pathways and molecular activities. These results provide an effec...