Preparation, Characterization, and Wound Healing Promotion of Hydrogels Containing Glucosyloxybenzyl 2-Isobutylmalates Extract from Bletilla striata (Thunb.) Reichb.f.

作者：Ran Fei, Kailang Mu, Gang Liu, Yuchen Liu, Yuxin Pang, Feng Guo, Lingli Zhou, Leqiang Peng · 发表于：International Journal of Molecular Sciences · 年份：2024 · DOI：10.3390/ijms251910563 · 被引用次数：8 · 研究领域：Biological and pharmacological studies of plants、Wound Healing and Treatments、Essential Oils and Antimicrobial Activity

(Thunb.Reichb.f.), specifically a glucosyloxybenzyl 2-isobutylmalates extract (B), and characterize its potential effects on wound healing. We synthesized the hydrogel using carbomer (C), glycerol (G), and triethanolamine (T) as the matrix, incorporating B into the hydrogel base, and evaluated its physical and chemical properties. In vitro tests assessed the biocompatibility of the glucosyloxybenzyl 2-isobutylmalates-carbomer-glycerol-triethanolamine (B-CGT) hydrogel and its effects on cell proliferation, migration, and adhesion. Animal model experiments evaluated its potential to promote wound healing. The results showed that the prepared B-CGT hydrogel possessed a good three-dimensional network structure and stability, demonstrating significant free radical scavenging capacity in antioxidant tests. In cell experiments, the B-CGT hydrogel exhibited no potential cytotoxicity and showed good hemocompatibility and promotion of cell proliferation. Animal experiments indicated that wounds treated with the B-CGT hydrogel healed significantly faster, with improved formation of new epithelial tissue and collagen. This study suggests that the developed B-CGT hydrogel is a promising candidate for wound dressings, with excellent physicochemical properties and controlled drug release capabilities, effectively promoting the wound healing process.