Gut microbiota and inflammation analyses reveal the protective effect of medium-chain triglycerides combined with docosahexaenoic acid on cognitive function in APP/PS1 and SAMP8 mice

作者：Zehao Wang, Yue Sun, Dalong Zhang, Yue Wang, Dezheng Zhou, Wen Li, Jing Yan, Yongjie Chen, Suhui Luo, Zhiyong Qian, Zhenshu Li, Guowei Huang · 发表于：Nutrition Research · 年份：2024 · DOI：10.1016/j.nutres.2024.09.015 · 被引用次数：4 · 研究领域：Diet and metabolism studies、Gut microbiota and health、Tryptophan and brain disorders

• Medium-chain triglycerides (MCTs) combined with DHA improve cognitive function. • 16S rRNA shows that MCTs combined with DHA optimize gut microbiota composition . • Proteobacteria and Firmicutes / Bacteroidota (F/B) ratio are linked with inflammation. • MCTs combined with DHA down-regulate the abundance of Proteobacteria and F/B ratio. Accumulating evidence has demonstrated that medium-chain triglycerides (MCTs) and docosahexaenoic acid (DHA) positively affect cognitive function. However, it remains unclear whether the improvement is related to the alterations of gut microbiota and inflammation and the impact of the combined intervention. In this study, we hypothesized that the supplementation of MCTs combined with DHA could modulate gut microbiota, inflammation, and improve cognitive function in APPswe/PS1De9 model mice and senescence-accelerated mouse-prone-8, which are two different mouse models used in neurodegeneration research. The mice were divided into four groups: Control group, MCTs group, DHA group, and MCTs + DHA group. The study assessed cognitive function, inflammatory cytokines, and gut microbiota composition. The results showed that supplementation of MCTs + DHA improved spatial learning ability, memory capacity, exploratory behavior; decreased the relative abundance of Proteobacteria ; reduced the ratio of Firmicutes / Bacteroidetes ; decreased the concentrations of serum interleukin (IL)-2, IL-6, monocyte chemotactic protein-1, tumor necrosis factor-alpha,...