Study on sperm cryopreservation of hybrid fish derived from Carassius cuvieri (♀) × Carassius auratus red var (♂)

作者：Qianqian Zeng, Yixuan Chen, Minyi Wang, Yinggang Li, Tao Dai, Weiling Qin, Yating Zhu, Chun Zhang, Yi Zhou, Qinbo Qin, Conghui Yang, Qianhong Gu · 发表于：Reproduction and Breeding · 年份：2024 · DOI：10.1016/j.repbre.2024.09.002 · 被引用次数：4 · 研究领域：Reproductive biology and impacts on aquatic species、Sperm and Testicular Function、Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities

Cryopreservation of sperm is an effective method for conserving germplasm resources in fish genetic breeding. The high-quality hybrid fish (WR) derived from white crucian carp ( Carassius cuvieri , WCC, ♀) and red crucian carp ( C . auratus red var., RCC, ♂), possesses valuable traits such as high survival rates, strong resistance, and rapid growth, representing an important germplasm resources of crucian carp. This study compared the effects of different antifreeze solutions on sperm viability among three varieties (WR, WCC, and RCC) and examined changes in enzyme activity, fertilization rates, and hatching rates after cryopreservation, aiming to enhance the cryogenic sperm cryopreservation technique in hybrid fish and investigate the mechanisms underlying spermatozoa damage caused by cryopreservation. The results showed that the antifreeze combination of D14 with 15 % dimethyl sulfoxide (DMSO) had the best effect in preserving the sperm of WR and WCC, while D20 with 10 % DMSO was the optimal combination for RCC sperm. After ultra-low temperature preservation, the longevity, fertilization, and hatching rates of frozen sperm were significantly lower ( P < 0.05) compared to fresh sperm. The enzyme activities of superoxide dismutase (SOD), lactate dehydrogenase (LDH), and creatine kinase (CK) were significantly decreased ( P < 0.05) in spermatozoa, whereas they showed a significant increase ( P < 0.05) in sperm plasma. Succinate dehydrogenase (SDH) activity was significantly re...