Increased intracellular H 2 S levels enhance iron uptake in Escherichia coli

作者：Shouta Nonoyama, Shintaro Maeno, Yasuhiro Gotoh, Ryota Sugimoto, Kan Tanaka, Tetsuya Hayashi, Shinji Masuda · 发表于：mBio · 年份：2024 · DOI：10.1128/mbio.01991-24 · 被引用次数：5 · 研究领域：Sulfur Compounds in Biology、Polyamine Metabolism and Applications、Amino Acid Enzymes and Metabolism

ABSTRACT We investigated the impact of intracellular hydrogen sulfide (H 2 S) hyperaccumulation on the transcriptome of Escherichia coli . The wild-type (WT) strain overexpressing mstA , encoding 3-mercaptopyruvate sulfur transferase, produced significantly higher H 2 S levels than the control WT strain. The mstA -overexpressing strain exhibited increased resistance to antibiotics, supporting the prior hypothesis that intracellular H 2 S contributes to oxidative stress responses and antibiotic resistance. RNA-seq analysis revealed that over 1,000 genes were significantly upregulated or downregulated upon mstA overexpression. The upregulated genes encompassed those associated with iron uptake, including siderophore synthesis and iron import transporters. The mstA -overexpressing strain showed increased levels of intracellular iron content, indicating that H 2 S hyperaccumulation affects iron availability within cells. We found that the H 2 S-/supersulfide-responsive transcription factor YgaV is required for the upregulated expression of iron uptake genes in the mstA -overexpression conditions. These findings indicate that the expression of iron uptake genes is regulated by intracellular H 2 S, which is crucial for oxidative stress responses and antibiotic resistance in E. coli . IMPORTANCE H 2 S is recognized as a second messenger in bacteria, playing a vital role in diverse intracellular and extracellular activities, including oxidative stress responses and antibiotic resista...