Sea conch ( Rapana venosa ) peptide hydrolysate regulates NF ‐ κB pathway and restores intestinal immune homeostasis in DSS ‐induced colitis mice

作者：Hidayat Ullah, Yamina Alioui, Muhsin Ali, Sharafat Ali, Nabeel Ahmed Farooqui, Nimra Zafar Siddiqui, Duaa M. Alsholi, Muhammad Ilyas, Mujeeb Ur Rahman, Yi Xin, Liang Wang · 发表于：Food Science & Nutrition · 年份：2024 · DOI：10.1002/fsn3.4410 · 被引用次数：4 · 研究领域：Protein Hydrolysis and Bioactive Peptides、Biochemical effects in animals

Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract. Sea conch peptide hydrolysate (CPH) was produced by enzymatic digestion of fresh conch meat with trypsin enzyme. To analyze the molecular composition, functional groups, and structural morphology of the hydrolysate, we employed liquid chromatography-mass spectrometry (LC-MS), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Results confirmed that crude protein could be effectively digested by enzymes to generate peptides. In this study, we evaluated the bioactivities of CPH on dextran sulfate solution (DSS)-induced colitis in mice. The findings demonstrated that CPH supplementation improved body weight, food and water intake, and colon length. The therapeutic efficacy and immunoregulatory effect of CPH were further determined. Our results exhibited that CPH treatment significantly ameliorated pathological symptoms by enhancing intestinal integrity, mucin production, and goblet cell count. Moreover, the immunoregulatory effect of CPH on mRNA expression levels of different pro- and anti-inflammatory cytokines was determined. Results exhibited a decrease in the expression of pro-inflammatory cytokines and an increase in anti-inflammatory cytokines in the colon. Additionally, the CPH administration modulates the nuclear factor kappa B (NF-κB) pathway, preventing DNA damage and cell death. Assays for apoptosis and DNA damage revealed that CPH r...