Decoding the duration of fertility of laying chicken through phenotypic and proteomic evaluation

作者：Md. Ahsanul Kabir, Huijuan Ruan, Rong Li, Md. Abu Horaira, X. Wu, Long Wang, Yingjie Wang, Jianan Cai, Shuxia Han, Shijun Li · 发表于：British Poultry Science · 年份：2024 · DOI：10.1080/00071668.2024.2378479 · 被引用次数：1 · 研究领域：Genetic Mapping and Diversity in Plants and Animals、Peroxisome Proliferator-Activated Receptors、Genetic and phenotypic traits in livestock

1. This study determined the effective indicators and proteins involved in long-duration fertility (DF) in chickens.2. Three lines of Chinese Xinhua chickens (900) were compared using seven phenotypic trait indicators, and the best was determined based on repeatability value. Subsequently, differential expression analysis, functional annotation and protein-protein interaction (PPI) network analyses were performed to investigate the pathways and hub proteins. Finally, qPCR analysis was conducted to validate the expression of identified hub proteins, and functional annotation with previously published genes was performed to explain how hub proteins work to maintain the trait.3. The study found that the number of fertilised eggs (FN) and maximum fertilised eggs (MCF) were the most repeatable among the seven indicators. It identified 231 differentially expressed proteins, with 144 being down-regulated and 87 being up-regulated. The differentially expressed proteins exhibited high clustering within various cellular compartments, including the cytosol and cytoplasm and GTP binding. Multiple pathways were identified, including tight and adherens junctions, TGF-beta signalling, autophagy-animal, regulation of actin cytoskeleton and the ribosome that may regulate the trait. Three hub proteins, KRAS, RPL5 (p < 0.001), and HSPA4 (p < 0.01), were significantly differentially expressed between high and low DF groups.4. This study identified FN and MCF as effective indicators for addressin...