Light-induced remodeling of phytochrome B enables signal transduction by phytochrome-interacting factor

作者：Zhengdong Wang, Wenfeng Wang, Didi Zhao, Yanping Song, Xiaoli Lin, Meng Shen, Cheng Chi, Bin Xu, Jun Zhao, Xing Wang Deng, Jizong Wang · 发表于：Cell · 年份：2024 · DOI：10.1016/j.cell.2024.09.005 · 被引用次数：65 · 研究领域：Light effects on plants、Plant Molecular Biology Research、Photosynthetic Processes and Mechanisms

Phytochrome B (phyB) and phytochrome-interacting factors (PIFs) constitute a well-established signaling module critical for plants adapting to ambient light. However, mechanisms underlying phyB photoactivation and PIF binding for signal transduction remain elusive. Here, we report the cryo-electron microscopy (cryo-EM) structures of the photoactivated phyB or the constitutively active phyB Y276H mutant in complex with PIF6, revealing a similar trimer. The light-induced configuration switch of the chromophore drives a conformational transition of the nearby tongue signature within the phytochrome-specific (PHY) domain of phyB. The resulting α-helical PHY tongue further disrupts the head-to-tail dimer of phyB in the dark-adapted state. These structural remodelings of phyB facilitate the induced-fit recognition of PIF6, consequently stabilizing the N-terminal extension domain and a head-to-head dimer of activated phyB. Interestingly, the phyB dimer exhibits slight asymmetry, resulting in the binding of only one PIF6 molecule. Overall, our findings solve a key question with respect to how light-induced remodeling of phyB enables PIF signaling in phytochrome research.