Bacterial and fungal aerosols in poultry houses: PM2.5 metagenomics via single-molecule real-time sequencing

作者：Xilong Wang, Lei Chen, Guiwen Yang, Yumei Cai, Guanliu Yu · 发表于：Poultry Science · 年份：2024 · DOI：10.1016/j.psj.2024.104348 · 被引用次数：7 · 研究领域：Indoor Air Quality and Microbial Exposure、Odor and Emission Control Technologies、Advanced Chemical Sensor Technologies

Microbial aerosol contamination is a common problem in poultry farms, posing potential health risks to poultry and their caretakers. Exploring the distribution and diversity of the microbial community in poultry farm aerosols is crucial for effective mitigation strategies. The composition of bacterial and fungal aerosols is poorly understood, particularly the prevalence of potential pathogenic microorganisms in fine particulate matter (PM 2.5 ) in various types of poultry houses. In this study, 27 PM 2.5 samples were collected from 5 chicken houses and 4 duck houses in Shandong Province, China. Species-level diversity of bacterial and fungal components in PM 2.5 samples was determined using advanced single-molecule real-time sequencing ( SMRT ) technology, based on the 16S and internal transcribed spacer 1 ( ITS ) ribosomal genes. Microbial diversity and community composition varied significantly across the different poultry house. Notably, duck houses had higher concentrations ( p < 0.01) of PM 2.5 (92.8–143.1 μg/m 3 ) than chicken houses (42.0–56.4 μg/m 3 ). Furthermore, microbial variation in PM 2.5 samples was associated with the type of poultry facility. The predominant pathogenic microorganisms included Aspergillus sydowii, Penicillium sp., Aspergillus insolitus, Cladosporium sp., Aspergillus sp., Aspergillus pseudoglaucus, Cladosporium sp . C4092-2-PD1, and Colletotrichum sp., all of which were classified as second category of pathogens. Aspergillus sydowii and Penicil...