Metabologenomics-Inspired Discovery and Combinatorial Biosynthesis-Based Diversification of Fungal O -Glycosylated Depsides

作者：Yang Hu, Zhuo Shang, Yingying Chen, Li Feng, Kunlong Li, Hongjie Zhu, Ming Peng, Jiafan Yang, Cunlei Cai, Jianhua Ju · 发表于：Organic Letters · 年份：2024 · DOI：10.1021/acs.orglett.4c03024 · 被引用次数：8 · 研究领域：Microbial Natural Products and Biosynthesis、Fungal Biology and Applications、Plant biochemistry and biosynthesis

Through metabologenomics mining, we prioritized Exophiala xenobiotica SDU 039, a deep-sea sediment-derived fungus producing O -glycosylated depsides ( 1 – 9 ), including seven new species with varying aliphatic chains. Heterologous expression validated the exo gene cluster, and in vitro enzyme assays elucidated the function of glycosyltransferase ExoC. The chemical diversity of O -glycosylated depsides is expanded by combinatorial biosynthesis using homologues depside biosynthetic genes and in vitro transformation with ExoC and different sugars as substrate.