A dynamic regulome of shoot-apical-meristem-related homeobox transcription factors modulates plant architecture in maize

作者：Zi Wei Luo, Leiming Wu, Xinxin Miao, Shuang Zhang, Ningning Wei, Shiya Zhao, Xiaoyang Shang, Hongyan Hu, Jiquan Xue, Tifu Zhang, Fang� Yang, Shutu Xu, Lin Li · 发表于：Genome biology · 年份：2024 · DOI：10.1186/s13059-024-03391-8 · 被引用次数：14 · 研究领域：Plant Molecular Biology Research、Plant Gene Expression Analysis、Genetic Mapping and Diversity in Plants and Animals

BACKGROUND: The shoot apical meristem (SAM), from which all above-ground tissues of plants are derived, is critical to plant morphology and development. In maize (Zea mays), loss-of-function mutant studies have identified several SAM-related genes, most encoding homeobox transcription factors (TFs), located upstream of hierarchical networks of hundreds of genes. RESULTS: Here, we collect 46 transcriptome and 16 translatome datasets across 62 different tissues or stages from the maize inbred line B73. We construct a dynamic regulome for 27 members of three SAM-related homeobox subfamilies (KNOX, WOX, and ZF-HD) through machine-learning models for the detection of TF targets across different tissues and stages by combining tsCUT&Tag, ATAC-seq, and expression profiling. This dynamic regulome demonstrates the distinct binding specificity and co-factors for these homeobox subfamilies, indicative of functional divergence between and within them. Furthermore, we assemble a SAM dynamic regulome, illustrating potential functional mechanisms associated with plant architecture. Lastly, we generate a wox13a mutant that provides evidence that WOX13A directly regulates Gn1 expression to modulate plant height, validating the regulome of SAM-related homeobox genes. CONCLUSIONS: The SAM-related homeobox transcription-factor regulome presents an unprecedented opportunity to dissect the molecular mechanisms governing SAM maintenance and development, thereby advancing our understanding of maize ...