Comprehensive comparative analysis and development of molecular markers for Lasianthus species based on complete chloroplast genome sequences

作者：Yue Zhang, Meifang Song, Deying Tang, Xianjing Li, Niaojiao Xu, Haitao Li, Lu Qu, Yunqiang Wang, Cuiyun Yin, Lixia Zhang, Zhonglian Zhang · 发表于：BMC Plant Biology · 年份：2024 · DOI：10.1186/s12870-024-05383-z · 被引用次数：10 · 研究领域：Genomics and Phylogenetic Studies、Biological and pharmacological studies of plants、Chromosomal and Genetic Variations

BACKGROUND: Lasianthus species are widely used in traditional Chinese folk medicine with high medicinal value. However, source materials and herbarium specimens are often misidentified due to morphological characteristics and commonly used DNA barcode fragments are not sufficient for accurately identifying Lasianthus species. To improve the molecular methods for distinguishing among Lasianthus species, we report the complete chloroplast (CP) genomes of Lasianthus attenuatus, Lasianthus henryi, Lasianthus hookeri, Lasianthus sikkimensis, obtained via high-throughput Illumina sequencing. RESULTS: These showed CP genomes size of 160164-160246 bp and a typical quadripartite structure, including a large single-copy region (86675-86848 bp), a small single-copy region (17177-17326 bp), and a pair of inverted repeats (28089-28135 bp). As a whole, the gene order, GC content and IR/SC boundary structure were remarkably similar among of the four Lasianthus CP genomes, the partial gene length and IR, LSC and SSC regions length are still different. The average GC content of the CP genomes was 36.71-36.75%, and a total of 129 genes were detected, including 83 different protein-coding genes, 8 different rRNA genes and 38 different tRNA genes. Furthermore, we compared our 4 complete CP genomes data with publicly available CP genome data from six other Lasianthus species, and we initially screened eleven highly variable region fragments were initially screened. We then evaluated the identific...