Expression reduction and a variant of a P450 gene mediate chlorpyrifos resistance in Tetranychus urticae Koch

作者：Dandan Xu, Haojie Liao, Chao He, Ke Wang, Rui Dong, Youjun Zhang, Zhaojiang Guo, Xin Yang, Wen Xie, Neil Crickmore, Shaoli Wang · 发表于：Journal of Advanced Research · 年份：2024 · DOI：10.1016/j.jare.2024.09.015 · 被引用次数：13 · 研究领域：Insect-Plant Interactions and Control、Insect Resistance and Genetics、Insect Pest Control Strategies

INTRODUCTION: Understanding how insects and mites develop resistance to chlorpyrifos is crucial for effective field management. Although extensive research has demonstrated that T. urticae exhibits high resistance to chlorpyrifos, the specific resistance mechanism remains elusive. Investigating this mechanism could provide valuable insights for pest control strategies. OBJECTIVES: This study aimed to reveal the mechanism of chlorpyrifos resistance in T. urticae. METHODS: The expression level of the CYP392D8 gene in T. urticae strains were analyzed using real- time quantitative PCR and western blot techniques. Functional validation of CYP392D8 was conducted through RNAi and heterogeneous expression. The production of chlorpyrifos-oxon in both resistant and susceptible strains were quantified using LC-MS/MS. Furthermore, the metabolic capabilities of CYP392D8 variants were verified using HPLC-MS and molecular docking. RESULTS: The results showed the expression of CYP392D8 was reduced in some Chinese resistant populations and mites with knocked down CYP392D8 showed decreased susceptibility to chlorpyrifos. Chlorpyrifos-oxon, the active metabolite of chlorpyrifos, was generated when chlorpyrifos was incubated with recombinant CYP392D8 protein in vitro. And a higher efficiency of chlorpyrifos-oxon formation was observed with the CYP392D8-S variant from susceptible mites compared to the CYP392D8-R variant from resistant mites. After treatment with low doses of chlorpyrifos, suscept...