Umbilical cord blood-derived exosomes attenuate dopaminergic neuron damage of Parkinson's disease mouse model

作者：Junjie Ye, Xiaodong Sun, Qi Jiang, Jianjun Gui, Shenglan Feng, Bingqing Qin, Lixia Xie, Ai Guo, Jinju Dong, Ming Sang · 发表于：Journal of Nanobiotechnology · 年份：2024 · DOI：10.1186/s12951-024-02773-1 · 被引用次数：21 · 研究领域：Extracellular vesicles in disease、Mesenchymal stem cell research、Neuroinflammation and Neurodegeneration Mechanisms

BACKGROUND: Umbilical cord blood (UCB) is a rich source of multifunctional stem cells characterized by low immunogenicity. Recent research in the fields of aging and regenerative medicine has revealed the potential of human umbilical cord blood-derived exosomes (UCB-Exos) in promoting wound healing, anti-aging, and regeneration. However, their role in neurodegenerative diseases, specifically Parkinson's disease (PD), remains unexplored. This study investigates the potential therapeutic effects and underlying mechanisms of UCB-Exos on PD. METHODS: Large extracellular vesicles (LEv), Exos, and soluble fractions (SF) of human UCB plasma were extracted to investigate their effects on motor dysfunction of the MPTP-induced PD mouse model and identify the key components that improve PD symptoms. UCB-Exos were administered by the caudal vein to prevent or treat the PD mouse model. The motor function and pathological markers were detected. Differentially expressed gene and KEGG enrichment pathways were screened by transcriptome sequence. MN9D and SH-SY5Y cells were cultured and evaluated for cell viability, oxidative stress, cell cycle, and aging-related indexes by qRT-PCR, western blot, immunofluorescence, and flow cytometry. The protein expression level of the MAPK p38 and ERK1/2 signaling pathway was detected by western blot. RESULTS: We observed that LEv, Exos, and SF all exhibited potential in ameliorating motor dysfunction in MPTP-induced PD model mice, with UCB-Exos demonstrati...