PSVI-27 Syntheses of guanidinoacetate and creatine in maternal tissues and placentae of gestating gilts fed diets with or without supplemental creatine

作者：E. Connolly, Wenliang He, Arianna Lopez, M. Olivarez, Joseph C. Cain, Carli Lefevre, Alex Ross, Gregory A. Johnson, Fuller W. Bazer, Guoyao Wu · 发表于：Journal of Animal Science · 年份：2024 · DOI：10.1093/jas/skae234.802 · 研究领域：Muscle metabolism and nutrition、Pharmacological Effects and Assays

Abstract This study determined the effects of dietary supplementation with creatine to gestating gilts (n = 6) on their ability to form guanidinoacetate (GA) and creatine in placentae and maternal tissues. Gilts were assigned randomly to one of two dietary groups: control and creatine supplementation (Cr; n = 12 gilts/diet). Once estrus was detected (d 0), gilts were artificially inseminated 12 h and 24 h later. All gilts were individually housed and fed 0.9 kg of a corn- and soybean-based diet containing 14% crude protein (but no creatine or GA) twice daily. Gilts in the control and Cr groups received daily top dressing of 0 or 2 × 15 g Cr either between d 10 and 60 or between d 10 and 90 of gestation. There were 6 gilts per dietary treatment per gestational stage. On either d 60 or 90 of gestation, gilts were euthanized to collect maternal tissues and placentae. Each tissue (~150 mg) was incubated at 37 ℃ for 2 h in 2 mL of oxygenated (95% O2/5% CO2) Krebs bicarbonate buffer with or without a) 2 mM glycine plus 2 mM L-arginine or b) 0.2 mM GA plus 0.5 mM L-methionine (the source of S-adenosylmethionine). After the 2-h period of incubation, 0.2 mL of 1.5 M HClO4 was added to the medium. The acidified medium plus tissue was homogenized and the homogenizer rinsed with 1 mL water, followed by the addition of 0.1 mLl of 2 M K2CO3. The extract was analyzed for GA, creatine, and amino acids using HPLC. Results indicated that on both d 60 and 90 of gestation, a) GA was formed from ...