Exploring extrahepatic metastasis of hepatocellular carcinoma based on methylation driver genes and establishing a prognostic model for hepatocellular carcinoma

作者：ShiLing Huang, Yang Yang, BoShu Ji, Ubaid Ullah, Ram Prasad Chaulagain, Yingying Tian, Jiawei Qiu, FeiYang Gao, PengChao Deng, Hongliang Chen, Ji-Han Qi, Xueyu Cang, Lina Liu, Shizhu Jin · 发表于：Gene · 年份：2024 · DOI：10.1016/j.gene.2024.148937 · 被引用次数：2 · 研究领域：Epigenetics and DNA Methylation、Ferroptosis and cancer prognosis、Kruppel-like factors research

• Two methylation drivers of HCC extrahepatic metastasis (DHX58, EIF5A2) are associated with the prognosis of HCC patients. • The prognostic risk model constructed by DHX58 and EIF5A2 is an independent risk factor for the prognosis of HCC patients. • Significant differences in DHX58 and EIF5A2 expression between HCC tissues with and without extrahepatic metastasis. • A higher abundance of M0 macrophages is related to a poor prognosis in HCC samples. Hepatocellular carcinoma (HCC), the seventh most common cancer worldwide, is characterized by a high mortality rate, advanced diagnosis, and susceptibility to extrahepatic metastasis. Numerous studies have shown that DNA methylation is a crucial factor in epigenetic modifications and regulation of carcinogenesis. HCC patient data were sourced from the TCGA dataset as a training set, while GSE116174 was used as an external validation set for verification. Differential methylation and expression analyses were performed on HCC samples with and without extrahepatic metastasis. In the intersecting genes, the relationship between methylation and expression levels of the intersecting genes was analyzed. Genes with a correlation coefficient≥|0.30| and P <0.05 were identified as methylation driver genes. Cox regression analysis was conducted to identify genes associated with HCC prognosis and establish a risk score. Subsequently, a prognostic model was established and validated using Cox regression analysis incorporating the risk score and...