MdABCI17 acts as a positive regulator to enhance apple resistance to Botryosphaeria dothidea

作者：Ying Xiang, Yuwen Zhao, Jingjing Wu, Xue Bai, Chu‐Kun Wang, Chang-Ning Ma, Quan Sun, Da‐Gang Hu · 发表于：Molecular Breeding · 年份：2024 · DOI：10.1007/s11032-024-01501-9 · 被引用次数：7 · 研究领域：Plant Pathogens and Fungal Diseases、Plant Physiology and Cultivation Studies、Postharvest Quality and Shelf Life Management

The ATP-binding cassette (ABC) superfamily is involved in numerous complex biological processes. However, the understanding of ABCs in plant pathogen defense, particularly against Botryosphaeria dothidea , remains limited. In this study, we identified MdABCI17 that plays a positive role in apple resistance to B. dothidea . Overexpression of MdABCI17 significantly enhanced the resistance of apple calli and fruits to B. dothidea . Our findings revealed that the jasmonic acid (JA) content and the expression of genes associated with JA biosynthesis and signal transduction were higher in stable MdABCI17- overexpressing apple calli than that of wild-type after inoculation with B. dothidea . Similar results were obtained for apple fruits with transient overexpression of MdABCI17 . Our research indicates that MdABCI17 enhances apple resistance to B. dothidea through the JA signaling pathway. We further determined that MdABCI17 plays a crucial role in the apple’s response to JA signaling. Moreover, exogenous methyl jasmonate (MeJA) treatment significantly enhanced the effectiveness of MdABCI17 in boosting apple resistance to B. dothidea . We proposed a positive feedback regulatory loop between MdABCI17 -mediated apple resistance to B. dothidea and JA signal. In summary, our study offers new insights into the role of ABC superfamily members in the control of plant disease resistance.