Unveiling the deterministic dynamics of microbial meta-metabolism: a multi-omics investigation of anaerobic biodegradation

作者：Xingsheng Yang, Kai Feng, Shang Wang, Mengting Yuan, Xi Peng, Qing He, Danrui Wang, Wenli Shen, Bo Zhao, Xiongfeng Du, Yingcheng Wang, Linlin Wang, Dong Cao, Wenzong Liu, Jianjun Wang, Ye Deng · 发表于：Microbiome · 年份：2024 · DOI：10.1186/s40168-024-01890-1 · 被引用次数：53 · 研究领域：Microbial Community Ecology and Physiology、Wastewater Treatment and Nitrogen Removal、Microbial Metabolic Engineering and Bioproduction

Microbial anaerobic metabolism is a key driver of biogeochemical cycles, influencing ecosystem function and health of both natural and engineered environments. However, the temporal dynamics of the intricate interactions between microorganisms and the organic metabolites are still poorly understood. Leveraging metagenomic and metabolomic approaches, we unveiled the principles governing microbial metabolism during a 96-day anaerobic bioreactor experiment. During the turnover and assembly of metabolites, homogeneous selection was predominant, peaking at 84.05% on day 12. Consistent dynamic coordination between microbes and metabolites was observed regarding their composition and assembly processes. Our findings suggested that microbes drove deterministic metabolite turnover, leading to consistent molecular conversions across parallel reactors. Moreover, due to the more favorable thermodynamics of N-containing organic biotransformations, microbes preferentially carried out sequential degradations from N-containing to S-containing compounds. Similarly, the metabolic strategy of C18 lipid-like molecules could switch from synthesis to degradation due to nutrient exhaustion and thermodynamical disadvantage. This indicated that community biotransformation thermodynamics emerged as a key regulator of both catabolic and synthetic metabolisms, shaping metabolic strategy shifts at the community level. Furthermore, the co-occurrence network of microbes-metabolites was structured around mi...