Intraindividual variability of semen quality, proteome, and sncRNA profiles in a healthy cohort of young adults

作者：Oleg Sergeyev, Vitalik Bezuglov, Natalia Soloveva, Luidmila Smigulina, Tatiana Denisova, Yury Dikov, Victoria Shtratnikova, Nikita E. Vavilov, Paige L. Williams, Susan Korrick, Mary Lee, Victor G. Zgoda, Russ Hauser, Alexander Suvorov · 发表于：Andrology · 年份：2024 · DOI：10.1111/andr.13739 · 被引用次数：5 · 研究领域：Sperm and Testicular Function、Male Reproductive Health Studies、Testicular diseases and treatments

Abstract Background Within‐subject variability of semen parameters and molecular components of ejaculates in young men remains poorly understood. Objectives To investigate intraindividual variability (IIV) of semen parameters and molecular markers in repeated ejaculates from young men. Materials and methods Semen parameters were assessed in samples collected 6–8 days apart from 164 18–19‐year old participants of the Russian Children's Study, a prospective cohort. Subsets of paired samples were used for label‐free quantitation and targeted mass‐spectrometry of proteins in seminal plasma (SP) and seminal extracellular vesicles (EVs), and for small non‐coding RNA (sncRNA) profiling in EVs and spermatozoa using RNA‐seq. The mean difference between two ejaculates, within‐subject variation, intraclass correlation, and concordance correlation were used to assess IIV for all parameters. Low variability with high reproducibility and high reliability was considered if CV w < 15% and ICC > 0.90, respectively. Results Analytical variability was low for all investigated parameters in technical replicates. IIV was assessed for basic semen parameters and proteins in SPs and EVs: 319 and 777 proteins, respectively, using untargeted analysis; 9 and 10 proteins using targeted quantification. We also described the IIV for sncRNA, including microRNA, piwi‐interacting RNA, tRNA, and tRNA‐derived small RNA (tsRNA) in EVs (409 sncRNA and 78 tsRNA) and in spermatozoa (265 sncRNA and 15 tsRNA)....