QTL mapping to identify loci and candidate genes associated with freezing tolerance trait in Camelina sativa

作者：TM Shaikh, Mukhlesur Rahman, James V. Anderson, Jinita Sthapit Kandel, Jayant Roy, Justin N. Vaughn, Timothy P. L. Smith, Brian Abernathy, Andrew Z. Ontano, Barbara Dobrin, Kevin Dorn, David P. Horvath · 发表于：Industrial Crops and Products · 年份：2024 · DOI：10.1016/j.indcrop.2024.119562 · 被引用次数：5 · 研究领域：Lipid metabolism and biosynthesis、Genetic Mapping and Diversity in Plants and Animals、Sunflower and Safflower Cultivation

Lack of freezing tolerance is a major constraint for the production of agronomically important Brassica species, particularly in the Northern Great Plains (NGP) of the United States and Canada. However, within the Brassicaceae family, winter germplasm of camelina have shown excellent freezing tolerance and overwinter potential in the NGPs. Differences in freezing tolerance between a winter variety (Joelle) and a spring variety (C046) of camelina appear to be controlled by a small number of dominant or co-dominant genes. To unravel the genetic mechanisms for the differences in freezing tolerance, 254 Recombinant Inbred Lines (RILs) were developed using reciprocal crosses between these two camelina varieties. The RIL population was phenotyped at the F7 stage for freezing tolerance under controlled conditions and genotyped by whole-genome skim sequencing. A one-way ANOVA test revealed a significant ( P < 0.001) difference exists among the RILs for freezing tolerance. A significant and strong correlation (r = 0.60, P < 0.001) was also observed between freezing tolerance and flowering time, indicating that regulation of flowering time might also influence freezing tolerance in camelina. A de novo linkage map was constructed using 4507 SNP markers covering a total of 1208.5 cM map distance with an average of 0.3 cM distances between the markers, which formed 20 linkage groups representing the 20 chromosomes (Chr) of C. sativa . The QTL analyses using three different programs reveal...