Insight into endophytic microbial diversity in two halophytes and plant beneficial attributes of Bacillus swezeyi

作者：Lei Gao, Jinbiao Ma, Yin Huang, Murad Muhammad, Hai-Ting Lian, Vyacheslav Shurigin, Dilfuza Egamberdieva, Wen‐Jun Li, Li Li · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1447755 · 被引用次数：14 · 研究领域：Plant-Microbe Interactions and Immunity、Microbial Community Ecology and Physiology、Genomics and Phylogenetic Studies

This study utilized high-throughput sequencing to investigate endophytic bacteria diversity in halophytic plants Anabasis truncate (AT) and Anabasis eriopoda (AE) from the Aral Sea region. Following sequence processing, 356 Amplicon Sequence Variants (ASVs) were discovered. The abundance and variety of endophytic bacteria were higher in AT. Bacillota , Pseudomonadota , Actinomycetota , and Bacteroidota constituted the dominant in AE, whereas Pseudomonadota , Actinomycetota , Bacteroidota , and Chloroflexota constituted the dominant in AT. Biomarkers were identified through LEFSe analysis, showing host-specific patterns. PCoA indicated distinct bacterial community structures. Phylogenetic analysis revealed diverse endophytic bacteria, including potential novel taxa. PICRUSt2 predicted diverse functions for endophytic bacteria in halophytes, indicating recruitment of beneficial bacterial taxa to adapt to extreme hypersaline conditions, including plant growth-promoting, biocontrol, and halophilic/tolerant bacteria. Moreover, the evolutionary relationship, metabolic capabilities, and plant beneficial potentials of the Bacillus swezeyi strains, previously isolated from the above two halophytes, were analyzed using comparative genomic and physiological analysis. The B . swezeyi strains displayed versatile environmental adaptability, as shown by their ability to use a wide range of carbon sources and their salt tolerances. B . swezeyi possessed a wide range of enzymatic capabilities...