Glia control experience-dependent plasticity in an olfactory critical period

作者：Hans C. Leier, Alexander J Foden, Darren A Jindal, Abigail J Wilkov, Paola Van der Linden Costello, Pamela J. Vanderzalm, Jaeda Coutinho‐Budd, Masashi Tabuchi, Heather T. Broihier · 发表于：eLife · 年份：2024 · DOI：10.7554/elife.100989 · 被引用次数：8 · 研究领域：Olfactory and Sensory Function Studies、Neurobiology and Insect Physiology Research、Photochromic and Fluorescence Chemistry

Sensory experience during developmental critical periods has lifelong consequences for circuit function and behavior, but the molecular and cellular mechanisms through which experience causes these changes are not well understood. The Drosophila antennal lobe houses synapses between olfactory sensory neurons (OSNs) and downstream projection neurons (PNs) in stereotyped glomeruli. Many glomeruli exhibit structural plasticity in response to early-life odor exposure, indicating a general sensitivity of the fly olfactory circuitry to early sensory experience. We recently found that glia shape antennal lobe development in young adults, leading us to ask if glia also drive experience-dependent plasticity during this period. Here, we define a critical period for structural and functional plasticity of OSN-PN synapses in the ethyl butyrate (EB)-sensitive glomerulus VM7. EB exposure for the first 2 days post-eclosion drives large-scale reductions in glomerular volume, presynapse number, and post- synaptic activity. Crucially, pruning during the critical period has long-term consequences for circuit function since both OSN-PN synapse number and spontaneous activity of PNs remain persistently decreased following early-life odor exposure. The highly conserved engulfment receptor Draper is required for this critical period plasticity as ensheathing glia upregulate Draper, invade the VM7 glomerulus, and phagocytose OSN presynaptic terminals in response to critical-period EB exposure. Loss ...