Identifying the physiological traits associated with DNA marker using genome wide association in wheat under heat stress

作者：Adeel Khan, Munir Ahmad, Muhammad Yousaf Shani, Muhammad Kashif Riaz Khan, Mehdi Rahimi, Daniel K. Y. Tan · 发表于：Scientific Reports · 年份：2024 · DOI：10.1038/s41598-024-70630-0 · 被引用次数：11 · 研究领域：Genetics and Plant Breeding、Wheat and Barley Genetics and Pathology、Genetic Mapping and Diversity in Plants and Animals

Heat stress poses a significant environmental challenge that profoundly impacts wheat productivity. It disrupts vital physiological processes such as photosynthesis, by impeding the functionality of the photosynthetic apparatus and compromising plasma membrane stability, thereby detrimentally affecting grain development in wheat. The scarcity of identified marker trait associations pertinent to thermotolerance presents a formidable obstacle in the development of marker-assisted selection strategies against heat stress. To address this, wheat accessions were systematically exposed to both normal and heat stress conditions and phenotypic data were collected on physiological traits including proline content, canopy temperature depression, cell membrane injury, photosynthetic rate, transpiration rate (at vegetative and reproductive stage and 'stay-green'. Principal component analysis elucidated the most significant contributors being proline content, transpiration rate, and canopy temperature depression, which exhibited a synergistic relationship with grain yield. Remarkably, cluster analysis delineated the wheat accessions into four discrete groups based on physiological attributes. Moreover, to explore the relationship between physiological traits and DNA markers, 158 wheat accessions were genotyped with 186 SSRs. Allelic frequency and polymorphic information content value were found to be highest on genome A (4.94 and 0.688), chromosome 1A (5.00 and 0.712), and marker Xgwm44 (...