HcLEA113 , a late embryogenesis abundant protein gene, positively regulates drought‐stress responses in kenaf

作者：Dengjie Luo, Caijin Wang, Samavia Mubeen, Muzammal Rehman, Shan Cao, Jiao Yue, Jiao Pan, Gang Jin, Ru Li, Tao Chen, Peng Chen · 发表于：Physiologia Plantarum · 年份：2024 · DOI：10.1111/ppl.14506 · 被引用次数：8 · 研究领域：Plant Stress Responses and Tolerance、Plant Molecular Biology Research、Plant responses to water stress

Abstract Late embryogenesis abundant (LEA) proteins have been widely recognized for their role in various abiotic stress responses in higher plants. Nevertheless, the specific mechanism responsible for the function of LEA proteins in plants has not yet been explored. This research involved the isolation and characterization of HcLEA113 from kenaf, revealing a significant increase in its expression in response to drought stress. When HcLEA113 was introduced into yeast, it resulted in an improved survival rate under drought conditions. Furthermore, the overexpression of HcLEA113 in tobacco plants led to enhanced tolerance to drought stress. Specifically, HcLEA113 ‐OE plants exhibited higher germination rates, longer root lengths, greater chlorophyll content, and higher relative water content under drought stress compared to wild‐type (WT) plants, while their relative conductivity was significantly lower than that of WT plants. Further physiological measurements revealed that the proline content, soluble sugars, and antioxidant activities of WT and HcLEA113 ‐OE tobacco leaves increased significantly under drought stress, with greater changes in HcLEA113 ‐OE plants than WT. The increase in hydrogen peroxide (H 2 O 2 ), superoxide anions (O 2 − ), and malondialdehyde (MDA) content was significantly lower in HcLEA113 ‐OE lines than in WT plants. Additionally, HcLEA113 ‐OE plants can activate reactive oxygen species (ROS)‐ and osmotic‐related genes in response to drought stress. On ...