Fruits from Rosa roxburghii : A Valuable Bioresource of Potent Radical Scavengers and Novel Ursane-Type Triterpenoids

作者：Yang Yu, Jing Wu, Mei-Fen Bao, Yang Liu, Zhi-Lin Cai, Johann Schinnerl, Xiang‐Hai Cai · 发表于：ACS Omega · 年份：2024 · DOI：10.1021/acsomega.4c04893 · 被引用次数：5 · 研究领域：Natural product bioactivities and synthesis、Phytochemicals and Antioxidant Activities、Antioxidant Activity and Oxidative Stress

High Resolution Image Download MS PowerPoint Slide Four new ursane-type triterpenoids named rosaroxine A–D and 21 known compounds were identified from Rosa roxburghii fruits. The structures of all compounds were established by 1D and 2D NMR spectroscopy and mass spectrometry. The phenolics catechin (EC 50 13.4 μM), quercetin (13.1 μM), gallic acid (10.0 μM), and protocatechuic acid (15.2 μM) were identified as powerful in vitro antioxidants with EC 50 values lower than ascorbic acid (31.3 μM). The triterpenoids rosaroxine C (EC 50 37.4; 40.3 μM) and 2-oxo-pomolic acid (16.6; 28.2) and the phenolics catechin (53.3; 29.0), quercetin (18.8; 33.1), and gallic acid (26.3; 40.0) exerted partly higher activities in the cyclo-oxygenase (COX 1/2) assay than the positive control acetaminophen (EC 50 45.0; >100 μM). The triterpenoids rosaroxine C and 2-oxo-pomolic acid also performed well in the anti-aging assay using HaCaT cells. Quantification of the bioactive compounds by LC-MS revealed concentrations of 3.08 mg kg –1 rosaroxine C, 17.40 mg kg –1 2-oxo-pomolic acid, 76.29 mg kg –1 catechin, and 5.58 mg kg –1 protocatechuic acid in the dried fruits. Overall, this work provides detailed phytochemical information, and the results from the accomplished bioassay point toward health promoting properties of these fruits.