Confinement Effect Enhanced Bipolar Electrochemistry: Structural Color Coding Coupled with Wireless Electrochemiluminescence Imaging Technology

作者：Xiaoyan Wang, Sheng-Tong Wu, Yi-Zhi Lin, Shou‐Nian Ding, Jing‐Juan Xu, Hong‐Yuan Chen · 发表于：Analytical Chemistry · 年份：2024 · DOI：10.1021/acs.analchem.4c01094 · 被引用次数：9 · 研究领域：Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection、Electrochemical Analysis and Applications

In this work, SiO 2 /CNTs photonic crystal beads were constructed by doping CNTs into SiO 2 photonic crystals, which have an angle-independent responsive structural color and can be used as bipolar electrodes due to their good electrical conductivity. In addition, the bipolar electrode-electrochemiluminescence (BPE-ECL) experiments and finite element simulation prove that the low driving voltage can trigger the bipolar electrode electrochemical reactions by confinement effect. Inspired by this, it is the first to combine the SiO 2 /CNTs structural color coding scheme with low-drive voltage induced wireless BPE-ECL imaging based on the confinement effect of microchannels to achieve simultaneous immune detection of ovarian cancer biomarkers (CA125, CEA, AFP). The detection limits of successfully constructed high-throughput BPE-ECL biosensor for AFP, CEA, and CA125 are 0.72 ng/mL, 0.95 ng/mL, and 1.03 U/mL, respectively, and have good stability and specificity, which expands the application of electrochemiluminescence and lays a foundation for the development of electrochemiluminescence coding technology.