Effect of exogenous melatonin on growth and antioxidant system of pumpkin seedlings under waterlogging stress

作者：Zhenyu Liu, Li Sun, Zhenwei Liu, Xin-Zheng Li · 发表于：PeerJ · 年份：2024 · DOI：10.7717/peerj.17927 · 被引用次数：12 · 研究领域：Plant responses to water stress、Innovations in Aquaponics and Hydroponics Systems、Magnetic and Electromagnetic Effects

Melatonin regulates defense responses in plants under environmental stress. This study aimed to explore the impact of exogenous melatonin on the phenotype and physiology of ‘BM1’ pumpkin seedlings subjected to waterlogging stress. Waterlogging stress was induced following foliar spraying of melatonin at various concentrations (CK, 0, 10, 100, 200, and 300 μmol·L −1 ). The growth parameters, malondialdehyde (MDA) content, antioxidant enzyme activity, osmoregulatory substance levels, and other physiological indicators were assessed to elucidate the physiological mechanisms underlying the role of exogenous melatonin in mitigating waterlogging stress in pumpkin seedlings. The results indicate that pumpkin seedlings exhibit waterlogging symptoms, such as leaf wilting, water loss, edge chlorosis, and fading, under waterlogging stress conditions. Various growth indicators of the seedlings, including plant height, stem diameter, root length, fresh and dry weight, and leaf chlorophyll content, were significantly reduced. Moreover, the MDA content in leaves and roots increased significantly, along with elevated activities of superoxide dismutase, catalase, peroxidase, and soluble protein contents. When different concentrations of melatonin were sprayed on the leaves post waterlogging stress treatment, pumpkin seedlings showed varying degrees of recovery, with the 100 μmol·L −1 treatment displaying the best growth status and plant morphological phenotypes. There were no significant diff...