Integrative analysis regarding the correlation between collagen-related genes and prostate cancer

作者：Yunfei Xiao, Cong Lai, Jintao Hu, Yelisudan Mulati, Xiaoting Xu, Jiawen Luo, Degeng Kong, Cheng Liu, Kewei Xu · 发表于：BMC Cancer · 年份：2024 · DOI：10.1186/s12885-024-12783-9 · 被引用次数：9 · 研究领域：Prostate Cancer Treatment and Research、Protease and Inhibitor Mechanisms、Connective Tissue Growth Factor Research

PURPOSE: Prostate cancer (PCa) is a common malignancy in men, with an escalating mortality rate attributed to Recurrence and metastasis. Recent studies have illuminated collagen's critical regulatory role within the tumor microenvironment, significantly influencing tumor progression. Accordingly, this investigation is dedicated to examining the relationship between genes linked to collagen and the prognosis of PCa, with the objective of uncovering any possible associations between them. METHODS: Gene expression data for individuals with prostate cancer were obtained from the TCGA repository. Collagen-related genes were identified, leading to the development of a risk score model associated with biochemical recurrence-free survival (BRFS). A prognostic nomogram integrating the risk score with essential clinical factors was crafted and evaluated for efficacy. The influence of key collagen-related genes on cellular behavior was confirmed through various assays, including CCK8, invasion, migration, cell cloning, and wound healing. Immunohistochemical detection was used to evaluate PLOD3 expression in prostate cancer tissue samples. RESULTS: Our study identified four key collagen-associated genes (PLOD3, COL1A1, MMP11, FMOD) as significant. Survival analysis revealed that low-risk groups, based on the risk scoring model, had significantly improved prognoses. The risk score was strongly associated with prostate cancer prognosis. Researchers then created a nomogram, which demonstrat...