Novel Self-Powered Biosensor Based on Au Nanoparticles @ Pd Nanorings and Catalytic Hairpin Assembly for Ultrasensitive miRNA-21 Assay

作者：Yan Shen, Futing Wang, Yujin Li, Jingxian Li, Xiang Shi, Yan Yang, Hongfen Yang, Ren Cai, Weihong Tan · 发表于：Analytical Chemistry · 年份：2024 · DOI：10.1021/acs.analchem.4c02751 · 被引用次数：22 · 研究领域：Advanced biosensing and bioanalysis techniques、Advanced Nanomaterials in Catalysis、Electrochemical sensors and biosensors

An ultrasensitive self-powered biosensor is constructed for miRNA-21 detection based on Au nanoparticles @ Pd nanorings (Au NPs@Pd NRs) and catalytic hairpin assembly (CHA). The Au NPs@Pd NRs possess excellent electrical conductivity to improve the electron transfer rate and show good elimination of byproduct H 2 O 2 to assist glucose oxidase (GOD) to catalyze glucose; CHA is used as an amplification strategy to effectively enhance the sensitivity of the biosensor. To further amplify the output signal, a capacitor is integrated into the self-powered biosensor. With multiple signal amplification strategies, the self-powered biosensor possesses a linear range of 0.1–10 –4 fM and a lower limit of detection (LOD) of 0.032 fM ( S / N = 3). In addition, the as-prepared self-powered biosensor displays potential applicability in the assay toward miRNA-21 in human serum samples.