Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide

作者：Xiaoli Wang, Ru-Nan Zhang, Yu-Lin Pan, Zhiming Li, Hong-Qiu Li, Ya-Ting Lei, Fang-Fang Zhao, Xiaoxiao Hao, Weiwei Ma, Cuiping Yu, Hongwei Yao, Xinyu Wang, Junjie Lv, Yong-Hui Wu, Shengyuan Wang · 发表于：Cell & Bioscience · 年份：2024 · DOI：10.1186/s13578-024-01285-7 · 被引用次数：6 · 研究领域：Potato Plant Research、Edible Oils Quality and Analysis、Sulfur Compounds in Biology

BACKGROUND: The impact of acrylamide (ACR) on learning and memory has garnered considerable attention. However, the targets and mechanisms are still unclear. RESULTS: Elongation factor 2 (eEF2) was significantly upregulated in the results of serum proteomics. Results from in vitro and in vivo experiments indicated a notable upregulation of Eukaryotic elongation factor 2 kinase (eEF2K), the sole kinase responsible for eEF2 phosphorylation, following exposure to ACR (P < 0.05). Subsequent in vitro experiments using eEF2K siRNA and in vivo experiments with eEF2K-knockout mice demonstrated significant improvements in abnormal indicators related to ACR-induced learning and memory deficits (P < 0.05). Proteomic analysis of the hippocampus revealed Lpcat1 as a crucial downstream protein regulated by eEF2K. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses indicated that eEF2K may play a role in the process of ACR-induced learning and memory impairment by affecting ether lipid metabolism. CONCLUSIONS: In summary, eEF2K as a pivotal treatment target in the mechanisms underlying ACR-induced learning and memory impairment, and studies have shown that it provides robust evidence for potential clinical interventions targeting ACR-induced impairments.