Identification and multimodal characterization of a specialized epithelial cell type associated with Crohn’s disease

作者：Jia Li, Alan J. Simmons, Caroline V. Hawkins, Sophie Chiron, Marisol Ramirez‐Solano, Naila Tasneem, Harsimran Kaur, Yanwen Xu, Frank Revetta, Paige N. Vega, Shunxing Bao, Can Cui, Regina N. Tyree, Larry Raber, Anna N. Conner, Jennifer M. Pilat, Justin Jacobse, Kara M. McNamara, Margaret M. Allaman, Gabriella Raffa, Alain P. Gobert, Mohammad Asim, Jeremy A. Goettel, Yash A. Choksi, Dawn B. Beaulieu, Robin Dalal, Sara Horst, Baldeep Pabla, Yuankai Huo, Bennett A. Landman, Joseph T. Roland, Elizabeth Scoville, David A. Schwartz, M. Kay Washington, Yu Shyr, Keith T. Wilson, Lori A. Coburn, Ken S. Lau, Qi Liu · 发表于：Nature Communications · 年份：2024 · DOI：10.1038/s41467-024-51580-7 · 被引用次数：52 · 研究领域：Inflammatory Bowel Disease、IL-33, ST2, and ILC Pathways、Immune cells in cancer

Crohn’s disease (CD) is a complex chronic inflammatory disorder with both gastrointestinal and extra-intestinal manifestations associated immune dysregulation. Analyzing 202,359 cells from 170 specimens across 83 patients, we identify a distinct epithelial cell type in both terminal ileum and ascending colon (hereon as ‘LND’) with high expression of LCN2, NOS2, and DUOX2 and genes related to antimicrobial response and immunoregulation. LND cells, confirmed by in-situ RNA and protein imaging, are rare in non-IBD controls but expand in active CD, and actively interact with immune cells and specifically express IBD/CD susceptibility genes, suggesting a possible function in CD immunopathogenesis. Furthermore, we discover early and late LND subpopulations with different origins and developmental potential. A higher ratio of late-to-early LND cells correlates with better response to anti-TNF treatment. Our findings thus suggest a potential pathogenic role for LND cells in both Crohn’s ileitis and colitis. Crohn’s disease (CD) is a complex disease associated with immune dysregulation. Here the authors use multimodal data to identify and characterize an epithelial cell population, termed ‘LND’ cells, in both terminal ileum and ascending colon, with LND interacting locally with immune cells and potentially contributing to CD pathology.