The potential of Gynostemma pentaphyllum in the treatment of hyperlipidemia and its interaction with the LOX1 ‐ PI3K ‐ AKT ‐ eNOS pathway

作者：Zhuyang Shen, Xin Gao, Dan Huang, Xiaojin Xu, Jianping Shen · 发表于：Food Science & Nutrition · 年份：2024 · DOI：10.1002/fsn3.4250 · 被引用次数：8 · 研究领域：Pharmacological Effects of Natural Compounds、Traditional Chinese Medicine Analysis、Ginseng Biological Effects and Applications

Abstract Gynostemma pentaphyllum , a traditional Chinese medicine, is widely used to treat various diseases, but its therapeutic effects and mechanisms of action on hyperlipidemia remain unclear. This study aims to investigate the effects of Danshen leaf on hyperlipidemia through network pharmacology, molecular docking, and cellular experiments, elucidating its multifaceted mechanism of action within the LOX1‐PI3K‐AKT‐eNOS pathway. First, the active ingredients and targets of G. pentaphyllum were screened using the Traditional Chinese Medicine Systems Pharmacology database. Then, targets for hyperlipidemia were identified using the OMIM and GeneCards databases, and potential therapeutic targets for G. pentaphyllum in treating hyperlipidemia were determined. An active ingredient‐target network was constructed using Cytoscape software, and a protein–protein interaction (PPI) network was built and visualized using the STRING database and Cytoscape software. Finally, GO functional and KEGG pathway enrichment analyses were performed, and the predicted mechanisms were validated through molecular docking and cell experiments. 85 targets for G. pentaphyllum and 1556 for Hyperlipidemia were screened, with 53 common targets. Twenty‐four active ingredients of G. pentaphyllum were found to be involved in the treatment of hyperlipidemia. Key nodes such as Rhamnazin, Isofucosterol, and quercetin, and targets NCOA2, NR3C2, PGR, and PPARG showed high relevance. In the PPI network, 8 nodes, i...