Transcriptomic analysis of Penaeus monodon in response to acute and chronic hypotonic stress

作者：Jing Ji, Qiaohuang Wang, Shuigen Li, Yanting Chen, Jiexin Zhang, Hanxiu Yu, Jinzhen Xu, Miaomiao Li, Renhao Zheng, Nan Lin, Ziping Zhang · 发表于：Frontiers in Veterinary Science · 年份：2024 · DOI：10.3389/fvets.2024.1464291 · 被引用次数：7 · 研究领域：Neurobiology and Insect Physiology Research、Invertebrate Immune Response Mechanisms、Physiological and biochemical adaptations

To investigate the different mechanisms of Penaeus monodon in response to acute and chronic hypotonic stress, RNA sequencing technology was employed to profile the gene expression patterns in the gill, hepatopancreas, and hemocyte at 0, 6, 48, and 72 h post acute hypotonic stress treatment (with salinity immediately decreased from 20 psu to 4 psu) and at 0, 2, 10, 15 days during chronic hypotonic stress treatment (with salinity gradually decreased from 20 psu to 4 psu). The control group (SC) was maintained at a constant salinity of 20 psu. Differentially expressed genes (DEGs) were identified, followed by further validation using real-time quantitative reverse transcription PCR (RT-qPCR). A total of 34,217 genes were expressed through sequencing. Compared with the control group, 8,503 DEGs were identified in the acute hypotonic stress group, comprising 3,266 up-regulated and 5,237 down-regulated genes. In the chronic hypotonic stress group, 8,900 DEGs were detected, including 3,019 up-regulated and 5,881 down-regulated genes. Gene Ontology (GO) functional annotation analysis indicated that DEGs were primarily enriched in biological processes such as cellular and metabolic processes, cellular components like membrane and other cellular components, and molecular functions including structural binding and catalytic activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that DEGs were predominantly concentrated in five major pathways: meta...