Characterization of water microbiota and their relationship with resident oysters during an oyster mortality event

作者：Mingkun Liu, Qingyuan Li, Wenwen Xu, Luping Wang, Fucun Wu, Lintao Tan, Li Li, Guofan Zhang · 发表于：Microbiology Spectrum · 年份：2024 · DOI：10.1128/spectrum.02881-23 · 被引用次数：7 · 研究领域：Marine Bivalve and Aquaculture Studies、Gut microbiota and health、Microbial Community Ecology and Physiology

ABSTRACT Microorganisms are vital for the health of marine invertebrates, and their assembly is driven by both deterministic and stochastic factors that regulate residents (innate to the host) and transients (from ambient water). However, the role of water microbiota and the significance of deterministic and stochastic processes in aquatic hosts facing mortality threats are largely unknown. This study examines the shifts in water microbiota during an oyster mortality event using amplicon sequencing and compared with those of resident oysters to disentangle the balance of the deterministic and stochastic factors involved. Water temperature and dissolved oxygen significantly shape the microbial community with a distinct monthly pattern, and Cyanobacteria blooms might exacerbate oyster mortality. The comparative analysis of microbial communities in oysters and water revealed that ≤ 21% of the genera were shared between oysters and water, implying that water microbiota cannot easily transfer into oysters. Furthermore, these shared genera had different functions, with oysters more involved in promoting host digestion and nutrient acquisition and water bacteria enriched more in functions promoting their own growth and survival. These findings illustrate that oysters may possess specific selection or barrier mechanisms that permit a small percentage of transients, controlled by stochastic factors and having a minimal effect on oyster mortality, to enter, whereas the majority of oyst...