Application of Second‐Generation Sequencing Technology in Lower Respiratory Tract Infection

作者：Chong Wang, Shuo Yang, Qi Liu, Hongchao Liu, Shangjia Jin, Jiajia Zheng, Xiumei Xiao, Xin Hou, Jing Li, Sisi Ma, Liyan Cui · 发表于：Journal of Clinical Laboratory Analysis · 年份：2024 · DOI：10.1002/jcla.25090 · 被引用次数：3 · 研究领域：Respiratory viral infections research、Gut microbiota and health、Pneumocystis jirovecii pneumonia detection and treatment

BACKGROUND: Lower respiratory tract infection (LRTI) has long been an important threat to people's life and health, so the rapid diagnosis of LRTI is of great significance in clinical treatment. In recent years, the development of the sequencing technology provides a new direction for the rapid diagnosis of LRTI. In this review, the advantages and disadvantages of second-generation sequencing techniques represented by metagenomics next-generation sequencing (mNGS) and droplet digital polymerase chain reaction (ddPCR) in LRTI were reviewed. Furthermore, it offers insights into the future trajectory of this technology, highlighting its potential to revolutionise the field of respiratory infection diagnostics. OBJECTIVE: This review summarises developments in mechanistic research of second-generation sequencing technology their relationship with clinical practice, providing insights for future research. METHODS: Authors conducted a search on PubMed and Web of Science using the professional terms 'Lower respiratory tract infection' and 'droplet digital polymerase chain reaction' and 'metagenomics next generation sequencing'. The obtained literature was then roughly categorised based on their research content. Similar studies were grouped into the same sections, and further searches were conducted based on the keywords of each section. RESULTS: Different studies discussed the application of second-generation sequencing technology in LRTI from different angles, including the detect...