High catalytic nickel-platinum nanozyme enhancing colorimetric detection of Salmonella Typhimurium in milk

作者：Jie Zhu, Lingyue Xu, Junlin Zhang, Yuxin Wang, Hongyue Yu, Chuanchuan Hao, Guohui Cheng, Daofeng Liu, Minghui Chen · 发表于：Journal of Dairy Science · 年份：2024 · DOI：10.3168/jds.2024-25111 · 被引用次数：7 · 研究领域：Advanced Nanomaterials in Catalysis、Biosensors and Analytical Detection、Advanced biosensing and bioanalysis techniques

Colorimetric qualitative and sensitive quantitative detection of Salmonella Typhimurium holds significant importance for ensuring food safety and preventing foodborne illnesses. In this study, an ultra-high catalytic activity and biocompatible nickel-platinum nanoparticle (NiPt NP) nanozyme is successful synthesized to prepare a nanozyme-linked immunosorbent assay (NLISA) strategy for the detection of Salmonella Typhimurium. The synthesized NiPt NP exhibit high oxidase-like catalytic efficiency, with a Michaelis-Menten constant of 0.493 m M , similar to that of natural horseradish peroxidase (HRP). The maximal reaction velocity was determined to be 1.97 × 10 −7 M ·s −1 , 1.97-fold higher than that of the HRP (1.0 × 10 −7 M ·s −1 ). Meanwhile, the antibody employed in this NiPt NP-based NLISA exhibits exceptional capture efficacy, generating a stable immune complex with Salmonella Typhimurium. The NiPt NP-based NLISA demonstrates sensitivity, specificity, convenience, and cost-efficiency for the detection of Salmonella Typhimurium. Under optimal conditions, this NiPt NP-based NLISA demonstrates a quantitative range of 10 3 ∼10 6 cfu/mL with a detection limit as low as 10 3 cfu/mL. A single-blind experimental testing detects different concentrations of Salmonella Typhimurium-spiked skim milk, indicating the application potential of the proposed NLISA in real samples. In all, this research provides novel insights into the synthesis of nanozymes with excellent catalytic activity ...