Bacillus velezensis YC89-mediated recruitment of rhizosphere bacteria improves resistance against sugarcane red rot

作者：Linyan Xie, Lufeng Liu, Yanju Luo, Xibing Rao, Shaozhi LV, Zhenfeng Qian, Yining Di, Hongbo Lou, Lilian He, Fusheng Li · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2024 · DOI：10.1186/s40538-024-00627-4 · 被引用次数：9 · 研究领域：Biofuel production and bioconversion、Plant-Microbe Interactions and Immunity、Enzyme Production and Characterization

Sugarcane red rot is a soil-borne disease caused by Colletotrichum falcatum. It can reduce the yield of sugarcane and the purity of sugarcane juice, which seriously restricts the development of sucrose industry. Biocontrol bacteria can control diseases by regulating rhizosphere microecology. In this study, the effects of biocontrol bacteria on sugarcane rhizosphere microecology were studied by metagenomics and metabolomics, and the control effects of biocontrol bacteria and rhizosphere dominant bacteria on sugarcane red rot were further explored by pot experiment. The results of metagenomic sequencing showed that inoculation with B. velezensis YC89 and pathogens could significantly change the microbial diversity of the sugarcane rhizosphere. The relative abundance of beneficial strains such as Streptomyces, Burkholderia, Sphingomonas, and Rhizobium increased significantly in the rhizosphere of sugarcane in the YC treatment group. Pseudomonas was significantly enriched in the rhizosphere of sugarcane in the C treatment group. The results of metabolome sequencing showed that the content of amino acids in sugarcane root exudates increased after inoculation with B. velezensis YC89, and the contents of phenolic acids and flavonoids decreased. Spearman correlation analysis showed that there was a significant correlation between differential metabolites and rhizosphere microorganisms. The results of pot experiment showed that YC89 strain and three rhizosphere microorganisms could si...